Dose Assessment Based On Molecular Biomarkers
W. F. Blakely, A. C. Miller, M. B. Grace, C. B. McLeland, J. M. Muderhwa, P. G. S. Prasanna
Our objectives are to identify and validate genes and their encoded proteins responsive to radiation exposure and to optimize analytical systems for biodosimetry applications. Preliminary research, using ex vivo human blood lymphocytes and in vivo murine blood model systems, focused on assessing the utility of the Haras proto-oncogene and targets associated with DNA repair. The effect of interindividual variation on the response of radiation-induced gene expression was evaluated using ex vivo peripheral lymphocytes obtained from multiple donors. We recently established a fluid-phase-optimized and quantitative real-time reverse transcriptase (RT-PCR) multiplex bioassay for measurement of candidate human radiation-responsive gene targets. Blood protein biomarkers, detected by conventional enzyme-linked immunosorbent assay (ELISA), are being transitioned for measurement by an analytical system based on a flow cytometry, multiple-target microsphere. In addition to enhancing emerging medical radiological response capabilities, these findings have applications in radiation therapy studies.
This abstract was presented at the 36th Annual Midyear Meeting, "Radiation Safety Aspects of Homeland Security and Emergency Response", Biophysical and Biological Techniques for Retrospective Radiation Dosimetry Session, 1/26/2003 - 1/29/2003, held in San Antonio, TX.